ABSTRACT
DREAM (downstream regulatory element antagonistic modulator) is a calcium-binding protein that regulates dynorphin expression, promotes potassium channel surface expression, and enhances presenilin processing in an expression level-dependent manner. However, no molecular mechanism has yet explained how protein levels of DREAM are regulated. Here we identified group I mGluR (mGluR1/5) as a positive regulator of DREAM protein expression. Overexpression of mGluR1/5 increased the cellular level of DREAM. Up-regulation of DREAM resulted in increased DREAM protein in both the nucleus and cytoplasm, where the protein acts as a transcriptional repressor and a modulator of its interacting proteins, respectively. DHPG (3,5-dihydroxyphenylglycine), a group I mGluR agonist, also up-regulated DREAM expression in cortical neurons. These results suggest that group I mGluR is the first identified receptor that may regulate DREAM activity in neurons.
Subject(s)
Calcium , Cytoplasm , Dynorphins , Methoxyhydroxyphenylglycol , Neurons , Potassium Channels , Presenilins , Proteins , Receptors, Metabotropic Glutamate , Up-RegulationABSTRACT
PURPOSE: To treat pupillary membrane which developed after cataract operation and vitrectomy,steroid eye drops with mydriatics or, in sever case, tPA has been used. However steroid eye drops needs prolonged treatment and tPA has many complications such as, infection, or hemorrhage. We tried to remove pupillary membrane with Nd-YAG laser by using peripheral quadrantic disruption technique. METHODS: We treated 8 eyes from Jan. 1996 to Oct. 1999, and observed within 24 hours following Nd-YAG laser treatment. The pupillary membrane was cut with Nd-YAG laser and subsequently absorbed within 24 hours. RESULTS: There was no such complicaitons, as increased IOP, hemorrhage, and IOL damage. Immediately after Nd-YAG laser, visual acuity was improved and fundus was examined. CONCLUSION: Therefore we recommend Nd-YAG laser membrenectomy in cases of pupillary membrane, which did not respond to steroid therapy.